Fig. 6. Expression of Egr1 in wounds. (A) A cDNA-array was probed with cDNA derived from 7-day-old wounds of transgenic and non-transgenic animals. Egr1 expression (arrows) was reduced 7 times in transgenic animals compared with non-transgenic animals. (B) Northern blot analysis for Egr1 using RNA form 13-day-old wounds. In normal skin, Egr1 expression (3.2 kb) is low, whereas in 13-day-old wounds Egr1 expression is elevated in comparison with unwounded skin only in non-transgenic animals. GAPDH expression (1.3 kb) was used as loading control. (C) Quantification of the northern blot analysis by PhosphorImager system. In 13-day-old wounds, the expression level of Egr1 is threefold higher in non-transgenic animals in comparison with unwounded skin. By contrast, no upregulation is found in transgenic animals.

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