Fig. 3. LET-502 and MEL-11 localize at cleavage furrows. (i) IF of LET-502, MEL-11
and DAPI in wild-type, let-502(sb106) or mel-11(it26)
embryos. (A-C) Arrows indicate that both LET-502 and MEL-11 are enriched at
the furrow during early stages of wild-type cleavage and are found throughout
the furrow as it ingresses (D-F). They remain at cell boundaries after cell
divisions are completed. (G,I) Cytoplasmic LET-502 is decreased in
let-502(sb106) mutant embryos. (H,I) MEL-11 staining is weakened in
let-502(sb106) in comparison with wild-type embryos and is almost
entirely depleted in mel-11(it26) embryos (K,L); however, LET-502
retained its location (J,L) in mel-11 mutants. Bar, 9 µm. (A-F)
n=21, (G,I) n=52, (H,I) n=40, (K,L) n=15,
(J,L) n=38. (ii) LET-502 and MEL-11 localization in wild-type and
let-502(sb106) embryos using digital deconvolution microscopy. Arrows
indicate the ingressing cleavage furrows. (A-C) LET-502 and MEL-11 overlap at
the ingressing furrow in wild-type, let-502(sb106) (D-F) and embryos
from let-502(ca201)/+ hermaphrodites (G-I). Bar, 6.5 µm. (A-C)
n=7, (D-F) n=4 and (G-I) n=4.
