Fig. 4. Localization of LET-502 with myosin (NMY-2) in wild-type embryos, and
localization of NMY-2 and actin in let-502 and mel-11 mutant
embryos using digital deconvolution microscopy. Arrows indicate the ingressing
or newly completed cleavage furrows. (A-C) LET-502 and NMY-2 colocalize at the
ingressing furrow during cleavage. (D-I) NMY-2 and actin still accumulate and
colocalize to the putative site of furrow formation in all of the
let-502(sb106) and let-502(sb106RNAi) embryos examined. Note
that NMY-2 also localizes to the spindle during metaphase (G). NMY-2 and actin
(J-L) and LET-502 and NMY-2 (M-O) retain their localization at the furrow in a
mel-11(it26) mutant embryo during cleavage. (A-C) Bar, 5 µm.
n=3 by deconvolution and n=13 by IF. (D-I) Bar, 7 µm.
n=11 let-502(sb106RNAi) stained for both NMY-2 and actin by
deconvolution and n=5 let-502(sb106RNAi) embryos stained for
NMY-2 only by IF. (J-L) n=4 stained for both NMY-2 and actin by
deconvolution, n=23 stained for NMY-2 only by IF and n=2
stained for actin only by IF. (M-O) n=2 by deconvolution and
n=23 by IF.
