Fig. 7. Mechanical restraint of the keratocyte cell body stimulates growth of
adhesion sites and penetration of microtubules into the lamellipodium. A trout
keratocyte cell body, injected with rhodamine vinculin, was arrested at time 0
by a micropipette (phase image). The fluorescence images and insets (of boxed
regions) are shown at the initiation of cell body arrest (0'0'')
and 1 minute later. Note the incorporation of vinculin into multiple, new
focal complexes in response to stress (at 1'00''). One example of
five cells is shown. (B) The same protocol was used as in A for a black molly
keratocyte injected with Cy-3 tubulin. The cell body was released 70 seconds
before the last frame (4'50''). The right panels show, in the
fluorescence channel, the regions boxed in the phase contrast images (left).
An example from 15 cells is shown.
