Fig. 1. cyk-3 mutant embryos fail to perform cytokinesis. (a)
cyk-3 embryos were dissected from homozygous mutant hermaphrodites,
and time-lapse microscopy was performed. During pronuclear migration,
cyk-3 embryos do not undergo any visible membrane contractions, and
they fail to perform pseudocleavage. At the time of anaphase, when wild-type
embryos initiate cytokinesis, cyk-3 embryos show no sign of
furrowing. At that stage, the mitotic spindle of both wild-type and
cyk-3 embryos is displaced towards the posterior hemisphere of the
cell. While nuclear division occurs with normal kinetics, cytokinesis does not
occur in these embryos. (b) Time-lapse analysis was performed on embryos
within the uterus of homozygous mutant hermaphrodites. As dissected embryos,
embryos inside the uterus do not show any furrowing at the time of anaphase.
Nuclear division, however, occurs normally.
