Fig. 11. Kinetics of endocytic `marker' association with phagosomes formed after
internalization of L. amazonensis amastigotes. IFN-
-treated
macrophages were infected at a multiplicity of four parasites/host cell. At
various times post-infection, they were processed as described in
Fig. 5 and the association of
the following molecules with parasite-containing compartments was examined:
transferrin receptor, EEA1, rab7p, macrosialin, lamp-1, cathepsin B and MHC
class II molecules. For each experiment and at each time point, the
percentages of parasite-containing organelles displaying the molecules listed
above were determined after counting about 100 organelles. Data represent the
means + or - range of two separate experiments.
