Fig. 12. Confocal microscopy analysis of the association of late endosome/lysosome
`markers' with early phagosomes formed after internalization of L.
amazonensis amastigotes. IFN-
-pre-treated macrophages were
infected (four parasites/host cell) and 30 minutes later processed for
immunofluorescence microscopy. Cell preparations were incubated with immune
sera or Abs directed against rab7p (A), lamp-1 (B) or cathepsin B (C) and then
with adequate fluorescein conjugates (green staining). In B and C, cells were
also stained with propidium iodide to visualize macrophage nuclei and parasite
nuclei and kinetoplasts (red staining). Optical sections (0.3-0.5 µm
thickness) are shown. The micrographs are representative of two separate
experiments. Bars, 2 µm.
