Fig. 2. F-actin accumulation around parasites during or following phagocytosis. (A)
Macrophages untreated (
) or treated with IFN-
(
, [UNK])
were infected with L. amazonensis metacyclic promastigotes (,
) or amastigotes ([UNK]) and then fixed and permeabilized at the
different time points indicated. F-actin was stained with Alexa Fluor 488
phalloidin (green staining) and parasites with a mouse
anti-Leishmania immune serum and a Texas Red conjugate (red staining)
before examination of the cells by fluorescence microscopy. The percentages of
promastigotes or amastigotes surrounded by F-actin were determined after
counting about 100 parasites for each time point. Data are the
means±s.d. of three experiments () or are from a single
experiment (,[UNK]). (B,C) Fluorescence confocal microscopy of
macrophages incubated for 10 minutes with L. amazonensis metacyclic
promastigotes (B) or amastigotes (C). F-actin and parasites were stained as in
A. A 3D reconstruction and an optical section (0.5 µm thickness) are shown
in B and C, respectively. Bar, 2 µm.
