Fig. 5. CENP-B and CENP-C define different alpha-satellite domains. Cell extracts
from crosslinked cells were treated for immunoprecipitation with pre-immune
serum (lane 2), anti-CENP-B (lane 3), anti-CENP-C (lane 4) or anti-ScII (lane
7) antibodies, or first immunodepleted for the DNA—CENP-B complexes and
then subjected to immunoprecipitation with anti-CENP-C (lane 5). As a control,
a sample immunodepleted with anti-CENP-B antibodies was subjected to a second
round of immunoprecipitation with the same antibody to verify the complete
depletion of the DNA—CENP-B complex (lane 6). Alpha-satellite DNA,
digested with EcoRI, was detected as described in
Fig. 2.
