Fig. 2. Preparation of recombinant proteins. (A) The recombinant protein units
DelN(1) (lane 1), DelNo(1) (lane 2), NoC(1) (lane 3), CP123(1) (lane 4),
E123(1) (lane 5), E3Ca(1) (lane 6), DelN(2) (lane 7), DelNo(2) (lane 8),
NoC(2) (lane 9), E3Ca(2) (lane 10) and oCP123(2) (lanes 11 and 12) were
resolved by SDS-PAGE under non-reducing (lane 11) or reducing conditions (all
other lanes) and stained with Coomassie blue. The asterisk marks the migration
position of DelN proteins. (B) Western blots of trimeric DelN(1) (lane 1) and
DelN(2) (lane 3), or monomeric DelNo(1) (lane 2) and DelNo(2), (lane 4),
resolved under non-reducing conditions, transferred to nitrocellulose and
probed with an antibody to TSP-1 (lanes 1,2) or an antibody to TSP-2 (lanes 3,
4). Molecular weight markers are indicated in kDa.
