Fig. 4. Cell attachment properties of trimerised TSP recombinant protein units. (A)
Attachment activity of trimeric TSP recombinant protein units. VSM cell
attachment was quantified on surfaces coated with 100 nM monomeric or trimeric
proteins as indicated and compared with the level of cell attachment to 100 nM
TSP-1. Bars show mean values ± s.e.m. from three experiments. (B)
Mechanisms of VSM cell attachment to the trimeric proteins, DelN(1), DelN(2)
and oCP123(1). Cells were incubated in the presence of 1 mM GRGDS peptide,
antibodies to integrin ß1, ß3, 
2 or 4 subunits (all at
5 µg/ml), 100 µg/ml heparin, 300 µg/ml chondroitin sulphate A or 1 mM
of CD47-binding peptide, KRFYVVMWKQVTQK, either alone or in combination. Cell
attachment was quantified after 1 hour of incubation and is expressed as the
percentage of cell attachment relative to untreated controls. Bars show mean
values ± s.e.m. from three experiments.
