. Spindle shortening (stages I and II) in MDCK expressing CaM-EGFP under vinblastine treatment (100 nM): projection of 7 deconvolved sections; time lapse 1 minute; 11 time points.

Redistribution of CaM-EGFP from the ‘ring-like’ structure in the polar region towards metaphase plate; first in big aggregates, the ‘star-like’ structures (stage III), then to small punctate shapes (stage IV), until eventually it is spread into the cytoplasmic mass going into stage V. MDCK, under vinblastine treatment (100 nM): projection of seven deconvolved sections; time lapse 2 minutes; 30 time points.

Subdivision of normal spindle pole into two and three CaM-containing paclitaxel-induced polar structures. 85-90% of the initial CaM fluorescence (after correction for photobleaching) was recovered in drug induced poles. The photobleaching of about 25% was evaluated from the fluorescent emission of the adjacent non-splitting centrosome. MDCK under paclitaxel treatment (30 nM): projection of 20 deconvolved sections; time lapse 2 minutes; 8 time points.