Fig. 2. Calmodulin distribution in the mitotic spindle in control cells and in mild
treatment with nocodazole. (A) Control metaphase HeLa (projection of
deconvolved sections). (B) Stage I: MDCK (nocodazole 0.01 µg/ml, 2 hours;
one section deconvolved). (C) Stage II: MDCK (nocodazole 0.01 µg/ml, 2
hours; projection of deconvolved section; adjacent panel shows only the
CaM-EGFP (green) of the same cell). In the control, as in the first two stages
of the mitotic spindle perturbation with depolymerising drug, CaM-EGFP is
located at polar level in a ring-like pattern, and in the mitotic spindle
along microtubules from pole to kinetochore with a decreasing intensity
(green, CaM-EGFP; red, 
-tubulin; blue, chromosomes). Bar, 10 µm.
