Fig. 3. Effect of nocodazole (6 hours, 0.1 µg/ml) on CaM-EGFP localisation in
MDCK cells. (A) Stage III of microtubule disruption; (i) star-like structure;
(ii) centrosomal remnant; (a1) centrosomal remnant; (a2)
star-like structure in control experiments with cold treatment (4°C, 15
minutes to 2 hours). The star-like substructure shows the CaM-EGFP
concentrated centrally with short microtubules radiating from it and the
chromosomal material present at the outermost region. The centrosomal remnants
show an alternating pattern of tubulin and CaM-EGFP with no obvious
colocalisation, seen as `ring' in projection. (B) Stage IV shows CaM location
at kinetochore level in the absence of residual microtubules as seen with
immunofluorescence. No centrosomal remnants could be seen (green, CaM-EGFP;
red, 
-tubulin; blue, chromosomes; projection of deconvolved series).
Bar, 10 µm.
