QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)

Fig. 1. Characterization of FO-1 cells expressing the FcRn ${alpha}$ -chain alone or in combination with ß2m. (A) Western blot analysis. Lysates of FO-1 cells (lane 1), FO-1 ß2m cells (lane 2), FO-1 cells expressing Myc-hFcRn (lane 3), and FO-1 ß2m cells expressing Myc-hFcRn (lane 4) were analyzed by SDS-PAGE and immunoblotting using polyclonal anti-FcRn peptide antibodies ( ${alpha}$ -FcRn; upper panel), monoclonal anti-Myc antibodies ( ${alpha}$ -Myc; middle panel), and polyclonal anti-ß2m antibodies ( ${alpha}$ -ß2m; lower panel). For reasons unknown, the anti-FcRn serum detected the ${alpha}$ -chain less well in lysates from FO-1 ß2m than in FO-1 cells. (B) Co-localization of the FcRn ${alpha}$ -chain and ß2m. FO-1 cells (a,c) and FO-1 ß2m cells expressing Myc-hFcRn (c,d,e) were fixed, permeabilized and stained with monoclonal anti-Myc ( ${alpha}$ -Myc; a,b) and polyclonal anti-ß₂m antibodies ( ${alpha}$ -ß₂-m; c,d). Panel e shows the merged staining for Myc-hFcRn (green) and ß₂m (red). (C) Co-immunoprecipitation of the FcRn ${alpha}$ -chain and ß₂m. FO-1 cells (lanes 1,5), FO-1 cells expressing Myc-hFcRn (lanes 2,6), FO-1 ß2m cells (lanes 3,7) and FO-1 ß2m cells expressing Myc-hFcRn (lanes 4,8) were lyzed and immunoprecipitated with monoclonal anti-Myc antibodies ( ${alpha}$ -Myc IP; lanes 1-4) or polyclonal anti-ß₂m antibodies ( ${alpha}$ -ß2m IP; lanes 5-8). Precipitated proteins were immunoblotted with polyclonal anti-Myc antibodies ( ${alpha}$ -Myc; top panel) and polyclonal anti-ß₂m antibodies ( ${alpha}$ -ß2m; bottom panel). The data in Fig. 1 is representative of at least three independent experiments carried out using two different cell clones.

Return to article