Fig. 4. Effects of HuD and ELAV ectopic expression on GFP reporter expression in
the third instar larval wing disc. The ectopic expression is achieved by
expressing either UAS-HuD or UAS-elav using
dpp-GAL4 as a driver. (A) Wild-type (Canton-S) wing disc shows
background level of fluorescence. (B) The expression pattern of
dpp-GAL4 driver in the wing disc is visualized using
UAS-GFP. (C-N) Effect of ectopic HuD (D,G,J,M) and ELAV (E,H,K,N) are
examined for UnGA (D,E), UnGH (G,H), UgGA (J,K) and
UgGH (M,N) expression. Basal GFP signal of UnGA (C),
UnGH (F), UgGA (I) and UgGH (L) is shown as
control. Ectopically expressed HuD has no discernable effect on either
UnGA (D) or UnGH (G) expression, while ectopic ELAV
expression leads to strong increase in GFP signal along dpp-GAL4
expression pattern for both reporters (E,H). On UgGA expression,
neither HuD nor ELAV have any obvious effect (J,K). In contrast, ectopic
expression of either HuD or ELAV strongly enhances UgGH expression
(M,N). For the genotype of each wing disc, see Materials and Methods.
