Fig. 5. Comparison of the effects of exposure to azide and of infection by an
adenovirus encoding ca-AMPK, or lacking AMPK, on hexose transport. Clone 9
cells in triplicate culture dishes were maintained uninfected (none) or
infected with adenovirus either encoding ca-AMPK (ca) or lacking the AMPK gene
(null) for 48 hours. The cells were washed twice with PBS and treated for 30
minutes with (azide) or without (basal) 5 mM azide. Uptake of
[3H]3-O-methyl-D-glucose was then measured as described in
the Materials and Methods. Results shown in (a) are means±s.e.m. (3).
In parallel, western blots of cell lysates (10 µg protein) were prepared as
described in the Materials and Methods section and stained with mouse
antibodies to c-myc (b). The mobilities of molecular weight markers are
indicated on the left. *This value is significantly different
(P<0.05) from basal uptake rate in non-infected cells and from
basal uptake rate in cells infected with adenovirus lacking the AMPK coding
region.
