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The spontaneous side of ryanodine receptors

Ryanodine receptors (RyRs), like IP₃ receptors, are ER Ca²⁺ channels that release Ca²⁺ into the cytosol in response to specific signalling pathways. In muscle and neurons, these channels localize to particular regions of specialized ER in order to generate specific Ca²⁺ signals; in other cells, such compartmentalization is less evident. To compare the intrinsic properties of RyR subtypes, Vincenzo Sorrentino and co-workers have expressed RyR1 and RyR3 in HEK 293 cells, which lack a specialized form of ER and do not normally express RyRs (see p. 2497). The authors find that, as expected, both RyR1 and RyR3 generate global ER Ca²⁺ signals in response to the agonist caffeine. Significantly, they also show that RyR3-expressing HEK 293 cells (but not those expressing RyR1) exhibit spontaneous, local Ca²⁺ transients, which are restricted to a few regions of the ER. This indicates not only that different RyR isoforms can generate distinct Ca²⁺ signals but that a channel itself, rather than a specific cellular environment, can confer spontaneous local Ca²⁺ release.

Related articles in JCS:

RyR1 and RyR3 isoforms provide distinct intracellular Ca²⁺ signals in HEK 293 cells

Daniela Rossi, Ilenia Simeoni, Marcella Micheli, Martin Bootman, Peter Lipp, Paul D. Allen, and Vincenzo Sorrentino
JCS 2002 115: 2497-2504. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content