Fig. 6. Effect of overexpression of N/S and A2G2 on myoblast
fusion and colocalization of MARCKS with filamentous actin. Myoblasts cultured
for 24 hours were transfected with the pcDNAs for Myc-tagged, wild-type MARCKS
(A-C), N/S (D-F) and A2G2 (G-I). These cells were
further cultured for the next 48 hours, followed by immunostaining with
anti-Myc antibody and then with FITC-conjugated anti-IgG antibody, as
described in Materials and Methods. They were also subjected to
rhodamine-phalloidin staining (C,F,I). The middle panels (B,E,H) show the
overlapped views of the left and right panels for colocalization of MARCKS and
F-actin. The extents of membrane fusion were 82%, 93% and 27% for the cells
transfected with the pcDNAs for Myc-tagged wild-type MARCKS, N/S and
A2G2, respectively. Magnification, 400x (A-F);
500x (G-I).
