Fig. 7. Effects of the N/S and A2G2 mutations on accumulation
of creatine kinase. Myoblasts that had been cultured for 24 hours were
transfected with recombinant pcDNAs for Myc-tagged wild-type MARCKS, N/S and
A2G2. After incubation for 48 hours, the cells were
stained with anti-Myc antibody (FITC) and anti-creatine kinase antibody (PE)
followed by FACS analysis, as described in Materials and Methods. The level of
creatine kinase (CK) in the cells transfected with pcDNA for the wild-type
MARCKS was expressed as 100% and the others were expressed as relative values.
Each of the data represent the mean±s.e. of triplicate
determinations.
