Fig. 4. Rac is involved in cortactin relocalization. HUVECs were electroporated
with the pMT90-myc Rac1 N17 expression vector. Eighteen hours after
electroporation, cells were detached, plated onto fibronectin-coated
coverslips and allowed to spread for 8 hours before overnight serum
starvation. Cells were then stimulated for 2 minutes with 0.5 µM S1P, fixed
and stained for detection of myc expression (left panel) and cortactin
localization (right panel). Arrows show myc-tagged RacN17-expressing
cells.
