Fig. 8. Src kinase inhibition does not block Rac activation by S1P. Serum-starved
HUVECs were pretreated with 5 µM PP2 for 20 minutes and 0.5 µM S1P was
added for the indicated times. Active, CRIB-bound Rac1 is shown in the upper
panel and the total amount of proteins in the middle panel. Fold stimulation
of GTP-bound Rac was determined following normalization against total Rac.
Blotting of the same samples with an anti-active-Erk1/2 antibody (pErk1/2) is
shown below. The results shown are representative of three independent
experiments.
