Fig. 2. Expression levels of GFP-Rab11 wt and mutants. Stably transfected K562
cells (1x105 cells) were lysed with PBS containing 1% Triton
X100. Samples were subjected to SDS-PAGE and transferred onto a nitrocellulose
membrane as described in the Materials and Methods. The membrane was incubated
with a rabbit anti-Rab11 serum and a corresponding HRP-labeled secondary
antibody and developed with a enhanced chemiluminescence detection kit
(Pierce). The intensity of the bands was quantified by densitometry and was
expressed as arbitrary units.
