Fig. 4. The large ring-shaped vesicles observed in the GFP-Rab11Q70L-transfected
cells are labeled with Bodipy-ceramide. Stably transfected cells
overexpressing the mutant GFP-Rab11Q70L were labeled with Lysotracker (Lys) or
monodansylcadaverine (MDC) as described in the Materials and Methods. Late
endosomes were labeled using an antibody against the mannose-6-phosphate
receptor (M6PR) and a Texas-Red-conjugated goat-anti rabbit secondary
antibody. Labeling with Bodipy-TR ceramide (B-Cer) was performed as described
in the Materials and Methods. Cells were mounted on coverslips and analyzed by
fluorescence microscopy. Images were obtained as described in
Fig. 3.
