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Fig. 2. Induction and purification of MBP-TbGpi8. Cells were induced using
0.3 mM IPTG and lysed by sonification. MBP-TbGpi8 was eluted with
elution buffer containing 10 mM maltose and analysed by SDS-PAGE. Lane 1,
molecular weight markers; lane 2, lysate from non-induced cells; lane 3, cells
induced with 0.3 mM IPTG; lane 4, MBP-TbGpi8 after purification using
an amylose column.
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