Fig. 5. Transamidase activity of TbGpi8 and MBP-TbGpi8. (a)
Trypanosomal lysate and substrate were incubated at 30°C in the presence
(
) or absence ([UNK]) of recombinant TbGpi8. (b) Recombinant
TbGpi8 () or MBP-TbGpi8 ([UNK]) and substrate were
incubated at 30°C. (c) Trypanosomal lysates were preincubated with
Gpi8-specific antibodies for 1 hour at room temperature before the enzyme
assay was performed. () control assay without antibodies; (
) 60
pg/ml antibody; (
) 120 µg/ml antibody, (
) 240 pg/ml antibody,
([UNK]) substrate alone.
