Fig. 7. Intracellular localization of TbGpi8 in bloodstream-form trypanosomes.
Affinity-purified chicken anti-TbGpi8 antibodies have been used to
localize TbGpi8 in intracellular compartments. This labelling was localized
specifically around the nucleus and in tubular and vesicular structures
throughout the cell. (a-d), trypanosomes were sequentially stained with
anti-Gpi8 antibodies and FITC-labelled anti-chicken IgG (a), anti-BiP
antibodies and TRITC-labelled anti-rabbit IgG (b) and bisbenzamide (c). (d)
shows an overlay of all images. (e) overlay of Gpi8- and BiP-stained cells.
Bars, 5 µm.
