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Fig. 7. Local expression of PtdIns4P 5-kinase (PI4P5K
) and local
activation of ERM proteins. HA-tagged PtdIns4P 5-kinase was
overexpressed in serum-starved A431 cells that were triple stained with
anti-HA mAb (A), anti-ERM pAb, TK89 (B) and anti-CPERM mAb (C).
PtdIns4P 5-kinase was occasionally concentrated on the surface of
vesicular structures (A) where ERM proteins were recruited (B) and
phosphorylated (C). Bar, 10 µm.
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