Fig. 1. Active Mek1 promotes NBT-II cell dissociation. (A) Cells were microinjected
into the nucleus with 100 ng/µl of constructs coding for MEK1SSDD (a,b),
CD2-p110 (c,d) and RalBV23 (e,f), incubated for 18 hours, fixed and double
labelled for the expression of the injected construct (a,c,e) and desmoplakin
(DP) (b,d,f). Arrows point to microinjected cells, and arrowheads indicate the
presence of desmoplakin immunostaining at the cell periphery. (B) For each
experiment, several coverslips were analysed and the percentage of
desmosome-negative cells was calculated as the ratio between the number of
cells without DP at the cell surface and the total number of cells. The
results have been averaged from several experiments (n>3) in which
at least 100 cells were microinjected. The means and s.e.m. are shown. Bar, 10
µm.
