Fig. 3. Raf-activating Ras mutants promote desmosome breakdown in NBT-II cells.
Cells seeded on coverslips were microinjected into the nucleus with 100
ng/µl of the indicated expression vectors. 18 hours later, they were fixed
and immunostained for both Ras and DP expression as described in the Materials
and Methods. For each experiment, several coverslips where analysed and the
percentages of desmosome-negative cells were calculated as in
Fig. 1B. The results have been
averaged for several experiments (n>3) in which at least 100 cells
were microinjected. The means and s.e.m. are shown.
