Fig. 1. Receptor molecules implicated in LPS-cellular activation are present in
lipid rafts. MonoMac-6 cells were treated with 1% Triton X-100 buffer for 1
hour on ice and then subjected to sucrose density gradient centrifugation.
Fractions were collected from the top of the gradient; 1%
n-octylglucoside was added to each fraction; and equivalent portions
of each fraction were analysed by SDS-PAGE and immunoblotting. The lipid raft
marker was detected using HRP-conjugated cholera toxin (A), the nitrocellulose
membranes were also probed with 26ic (CD14-specific mAb) (B), hsp70 (C), hsp90
(D), CXCR4 mAbs (E) and GDF5 polyclonal serum (F), as well as with the HTA125
TLR4-specific mAb (G). The relative positions of the raft and non-raft
(soluble) fractions are indicated.
