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Fig. 4. TLR4 and GM-1 ganglioside FRET mearurements. Energy transfer between TLR4 (FITC-HTA125) and GM-1 ganglioside (rhodamine-cholera-toxin) before (A,B) and after (C,D) LPS stimulation. Energy transfer can be detected by the increase in donor fluorescence after acceptor photobleaching. Donor (FITC) after (A,C) acceptor photobleaching and (B, D) E image. Bar, 10 µm.





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