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Fig. 5. MCD disrupts lipid raft formation. MonoMac-6 cells were either not treated
(A) or treated (B) with 10 mM MCD for 10 minutes, before solubilisation in 1%
Triton X-100 buffer, followed by raft and non-raft separation by sucrose
density gradient centrifugation. The GM-1 ganglioside distribution was
visualised using HRP-conjugated cholera toxin.
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