Fig. 7. Raft-disrupting drugs do not alter surface expression of molecules involved
in LPS-induced cellular activation. MonoMac-6 cells were either mock-treated
(A,C,E) or treated with 10 mM MCD (B,D,F) for 10 minutes before washing with
buffer. Surface expression of CD14 (A,B), CXCR4 (C,D) or TLR4 (E,F) was
determined by flow cytometry. All primary antibodies were visualised with
FITC-conjugated secondary antibodies and analysed by flow cytometry utilising
a FACScalibur (Becton Dickinson) counting 10,000 cells per sample.
