Fig. 1. The epidermis of adult K10-/- mice showed hyperkeratosis and
acanthosis; cells and nuclei were enlarged (B). Keratohyalin was increased (B,
inset, arrow) compared with that in the wild-type (A). A and B show
H&E-stained paraffin sections of ear skin. DAPI staining of normal ear
epidermis showed epidermal proliferative units (C, epidermal sheet, small
arrow; E, higher magnification, arrow; G, section, arrow; large arrow in C and
D, hair follicle), which were absent in knockout mice (D, epidermal sheet; F,
higher magnification of D; H, section). In normal epidermis Ki-67 was
restricted to basal cells (I), whereas it was expressed throughout the basal
layer and in two to three suprabasal layers in K10-/- mice (K). sb,
stratum basale; sg, stratum granulosum; ss, stratum spinosum. Bars, 40 µm.
The respective wild-type and knockout photos are of the same
magnification.
