Fig. 2. Western blotting showed that the amount of K1 was slightly increased in
K10-/- skin (A, lane 4) compared with that in wild-type skin (A,
lane 3; lanes1,2, corresponding Coomassie-stained blot). K5 (B, lane 4,
K10-/-; lane 3, wild-type; lanes 1,2, corresponding blot) and K14
(C, lane 4, K10-/-; lane 3, wild-type; lanes 1,2, corresponding
blot) were also slightly increased, whereas K15 was unchanged (D, lane 4,
K10-/-; lane 3, wild-type; lanes 1,2, corresponding blot).
Immunofluorescence analysis of ear epidermis showed unaltered suprabasal K1
localization (E, wild-type; F, K10-/-), K5 and K14 were detected in
basal and suprabasal layers (K10-/-, H and K, respectively;
wild-type, G and I). K15 remained restricted to the basal layer
(K10-/-, M; and wild-type, L). Bars, 50 µm.
