Fig. 5. Immunohistochemical analysis showed an increase in cyclin D1 (B) and c-Myc
(D) in K10-/- epidermis compared with the wild-type, where both
proteins are exclusively found in a restricted number of basal cells (A and C,
respectively). The expression of ß-catenin remained restricted to
keratinocyte cell membranes (E, wild-type; F, K10-/-). (G) Northern
blotting revealed that the expression of Wnt-3 (lane 3, wt; lane 4,
K10-/-; loading control: lane 1, wt; lane 2, K10-/-),
Wnt-4 (lane 7, wt; lane 8, K10-/-; loading control: lane 5, wt;
lane 6, K10-/-1) and Wnt-5a (lane 11, wt; lane 12,
K10-/-; loading control: lane 9, wt; lane 10, K10-/-)
were not altered in K10-/- skin. Arrowheads point to distinct RNA
species. Bar, 40 µm.
