Fig. 6. 14-3-3
was markedly increased in the cytoplasm of suprabasal
K10-/- keratinocytes (B) compared with the wild-type (A). (C)
Protein extracts of skin revealed a strong increase in 14-3-3
expression in K10-/- mice (lane 5) compared with the wild-type
(lane 4; lane 1, marker; lanes 2,3, Coomassie-stained blot of lanes 4,5; dots
from top to bottom: 36 kDa and 26 kDa). (D) Northern analysis showed an
equally strong increase in 14-3-3 mRNA (lane 4) over the wild-type
(lane 3; lanes 1,2, ethidium-stained agarose gel corresponding to lanes 3,4;
dots from top to bottom: 2.8, 1.8 and 1.5 kb). Bar, 40 µm.
