Fig. 8. The restriction patterns for NlaIII in exon 1 (A) and
BsaAI in exon 2 (B) of the p16Ink4a gene revealed the
presence of polymorphisms in K10-/- mice that were on a mixed
BALB/cx129/Ola background. Lanes 2,4,6,8 (A,B) show undigested PCR
products of the respective exons. p16 exon 1 of BALB/c mice (A, lane 3) showed
a different restriction pattern from the 129/Ola p16 gene (A, lane 5).
K10-/- mice as well as wild-type control animals of a mixed strain
background showed an intermediate pattern (A, lane 7 and lane 9,
respectively). Exon 2 of the BALB/c p16 gene (B, lane 3) lacked a restriction
site that was present in the 129/Ola p16 gene (B, lane 5). K10-/-
mice, as well as the wild-type mice of the same mixed strain background, also
showed an intermediate pattern (B, lane 7 and lane 9, respectively). Size
markers from top to bottom: (A) 200 bp, 100 bp and 80 bp; (B) 400 bp and 300
bp.
