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Fig. 2. Effects of PEX2 depletion on matrix protein import and glycosome morphology
in bloodstream form trypanosomes. Cells were grown without Tet (control, -Tet)
or in the presence of 100 ng/ml Tet for 24 hours (+Tet). For the top and
middle rows, ALD was stained with AlexaFluor 488 and PEX11 was detected by a
Cy3-coupled secondary antibody. In the bottom row, PEX11 was Alexa-stained and
HXK detected by Cy3. Corresponding Nomarski images and DAPI staining are shown
on the left. Bar, 10 µm.
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