Fig. 4. Internalization of Yersinia and invasin-attachment by GD25 cells
expressing different ß1A-integrin mutants. Cells were infected with
Yersinia at a calculated bacteria:cell ratio of 150:1 for 30 minutes
at 37°C, and thereafter fixed and stained for extracellular and total
cell-associated bacteria respectively. (A) The percentage of cell-associated
bacteria located intracellularly is shown. (B) The total number of bacteria
associated to one cell. The values represent the means±s.e.m. of at
least five separate experiments. (C) The indicated cells were allowed to bind
to microtiter plates coated with GST-invasin (solid line), GST alone (dotted
line) or vitronectin for 1 hour. The number of cells adhering to vitronectin
(coated at 10 µg/ml) represents 100% of attached cells.
