Fig. 1. Fak-/- MEFs are deficient for Y. pseudotuberculosis
uptake. (A) The percentage of internalized Y. pseudotuberculosis
observed in infected Fak+/+, Fak-/- and
Fak-/- MEFs expressing ectopic Fak is presented as the average of
eight experiments. Error bars indicate standard deviation from the mean. (B) A
representative immunoblot showing Fak expression from lysates derived from
Fak+/+, Fak-/-, and Fak-/- MEFs reconstituted
to express Fak. (C) Overlay of fluorescence images (400x magnification)
of infected Fak-/- MEFs, including one Fak-transfected cell (blue).
Intracellular Y. pseudotuberculosis appear red and extracellular
Y. pseudotuberculosis appear yellow.
