Fig. 4. Identification of genes expressed by arrested undifferentiated C2C12 muscle
cells. (A) Differential display PCR analysis. Expression profiles of RNA from
asynchronous (Mb) suspension-arrested (S) and differentiated (Mt) C2C12 cell
cultures displayed by differential display PCR. (Mb and S were analyzed in
duplicate.) The arrowhead indicates a fragment representing a cDNA expressed
in arrested myoblasts but not in either asynchronous or differentiated
cultures (see Fig. 4E). (B-E) Transcripts detected by differential display PCR
fragments are enriched in synchronized C2C12 cells. Fragments excised from
differential display PCR gels were labeled and used to probe 10 µg of total
RNA from asynchronous myoblasts (Mb), arrested myoblasts (S) and myotubes
(Mt). Each fragment (CF1-4) detected a unique transcript; all were expressed
preferentially in synchronized cells. The sizes of the transcripts (B-E) are
3.5 kb (CF1/matrilin2), 2.2 kb (CF2/Znf216), 1.7 kb (CF3/TTP) and 1.4 kb
(CF4/LIX). Positions of 28 and 18S rRNA are marked (-). L7 demonstrates equal
loading of RNA.
