Fig. 8. LIX and TTP transcripts are distributed in a pattern similar to MyoD in
injured skeletal muscle. RNA in situ hybridization to frozen sections (20
µm) of injured TA muscle using digoxigenin-labeled antisense probes to LIX,
MyoD and TTP transcripts (A, B and C, respectively). Absence of staining in
the myofiber interior suggests sequestration of the transcripts in mononuclear
cells at the myofiber periphery. The same probes show no hybridization to
uninjured muscle (a, b and c). LIX and MyoD were detected 6 hours post injury
and TTP at 3 hours post injury. (D) shows TTP-positive cells located in the
lesion; an adjacent uninjured area is devoid of TTP transcripts. Data are
representative of three independent experiments each involving multiple
cryosections from two mice.
