Fig. 6. Protein Kinase C isoform expression in NA and TA cells. (A) NA and TA cells
were serum starved for 2 days then left untreated or treated with 10 ng/ml EGF
for 5 minutes. Whole cell extracts were prepared and analysed by western
blotting using antibodies for PKC
, PKC
, PKC
and
PKC
. (B) Total RNA was prepared from NA and TA cells then 20 µg
samples were electrophoresed, blotted then probed with a radiolabelled PCR
fragment of human PKC. Equivalent loading of RNA samples was
demonstrated using a 7S ribosomal cDNA probe.
