Fig. 1. The formation of stress-fibre-like structures in BRG1-expressing clones.
Immunofluorescence images show a BRG1-expressing clone of SW13 cells seeded
for 2 days before being fixed and stained with affinity-purified anti-BRG1
antibodies against the N-terminal part of the protein visualised by
TRITC-conjugated secondary anti-rabbit antibodies (A) and FITC-conjugated
phalloidin (B) to visualise actin filament structures from the same cells. In
(C), an immunofluorescence image of a second BRG1-expressing clone grown for 5
days before fixation and staining with phalloidin is shown. Immunofluorescence
images of a BRG1-K798R-expressing clone stained with affinity-purified
anti-BRG1 antibodies as in A (D), and the same cells stained with phalloidin
(E). Immunofluorescence images of SW13 cells fixed and stained with
affinity-purified anti-BRG1 antibodies as in A (F), and the same cells stained
with phalloidin (G). Bar, 20 µm; the magnification is the same in A-G. (H)
Cell lysates were made from untransfected SW13 cells and from clones
expressing the BRG1 protein or the BRG1-K798R protein. The proteins (20
µg/sample) were separated on a 7% SDS-PAGE for detection of BRG1 and a 15%
SDS-PAGE for the detection of actin, and transferred to a membrane that was
subsequently probed with anti-BRG1 antiserum or monoclonal ß-actin
antibodies. The lane marked `sw13' is lysate from untransfected cells, `BRG1'
is from the BRG1-expressing clone and `K798R' is from the
BRG1-K798R-expressing clone.
