Fig. 2. The effect of a specific antisense BRG1-RNA on the formation of stress-fibre-like actin filament structures. Cells were transfected with a vector expressing a specific BRG1 antisense RNA and the green fluorescent protein (GFP) as a marker. (A) Cell lysates (20 µg/sample) from BRG1-expressing cells grown for 72 hours after transfection and from untransfected BRG1-expressing cells were separated using 7% SDS-PAGE and probed with anti-BRG1 serum (top) and anti--tubulin (bottom). The amounts of antisense vector (µg) used for transfection of 0.6x10⁶ BRG1-expressing cells are indicated above the lanes. (B) An immunofluorescence image shows BRG1-expressing cells transfected with the antisense BRG1-vector stained with TRITC-conjugated phalloidin. (C) The same cells visualised by GFP-fluorescence as a marker for transfected cells. BRG1-expressing cells were also transfected with the GFP-vector without the antisense insert and stained with TRITC-conjugated phalloidin (D). The same cells were visualised by GFP-fluorescence as a marker (E). Bar, 20 µm; the magnification is the same in B-E.

Return to article