Fig. 8. The effect of Rho-kinase/ROCK expression in SW13 cells. (A,B)
Immunofluoroscence images show SW13 cells expressing a myc-tagged,
constitutively active Rho-kinase/ROCK fragment habouring the kinase domain,
which were fixed and stained with FITC-phalloidin (A) and the anti-9E-myc
antibody (B) for detection of cells expressing the Rho-kinase/ROCK fragment.
Immunofluorescence images of cells expressing a wild-type, myc-tagged ROCK1
protein stained with FITC-phalloidin (C) and anti-9E-myc antibodies (D), and
SW13 cells expressing a wild-type, myc-tagged ROCK2 protein stained with
FITC-phalloidin (E) and anti-myc-9E-antibodies (F). Immuofluorescence images
of BRG1-expressing cells transfected with a dominant negative, myc-tagged
ROCK1 (KD-IA), stained with FITC-phalloidin (G) and anti-9E-myc antibodies
(H). Bar, 20 µm; the same magnification was used in A-H.
