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2. Histology of SPARC-null lens at E14 and E18. Sections of wt and SPARC-null lenses were stained with hematoxylin and eosin and were examined by light microscopy. The sizes of the SPARC+/+ and SPARC-null (-/-) lenses at E14 and E18 are highly similar. The primary lens fibers have elongated to contact the anterior epithelium, and the lens vesicle lumen has disappeared at E14. The -/- lens fiber elongation is complete, and there are no vacuoles in the -/- lenses. Bar (A,B), 270 µm; bar (C,D), 400 µm.





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