Fig. 8. Recruitment of cIAP1 and cIAP2 into the TNF-R2 complex critically depends
on TRAF2 in living cells. HeLa-TNF-R2 cells were transfected with expression
plasmids encoding for cIAP1(NT)-GFP (A) or cIAP1(NT)-GFP (B), alone or in
combination with an expression construct for full-length TRAF2. The following
day, distribution of the GFP chimeras was both analyzed prior to stimulation
(2 µg/ml 
TNF-R2 IgG) and monitored online for 15 minutes after
stimulation using confocal microscopy. Examples of ligand-induced alterations
in the localization of the GFP fusion proteins are indicated with arrows.
